Phenol Chloroform protocol

Molecular Biology product line

G-Microparticles
Quick-to-Use product

Pretreated, sterile and neutral glass microparticles, ready to use. For general applications in Cellular and Molecular Biology, mainly in cell lysis protocols of wall resistant organisms, such as plants, fungi and yeasts. They are used as pre-extraction step for proteins, DNA and RNA.

Size ranges
1) extralarge, from 350 a 700 µm (cod 0140-XL)
2) large, from 180 a 425 µm (cod 0140-L)
3) medium, from 100 a 200 µm, (cod 0140-M)
4) small, from 50 to 100 µm, (cod 0140-S)

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Quick-Page
Quick-to-Use product

Kit to prepare SDS-PAGE gels in denaturalization conditions. DTT and 2-mercapto ethanol are not included.

» Acrylamide / bisacrylamide (Premix) 29:1, 100 ml
» Separation buffer 4X, 70 ml
» Stacking buffer 4X, 35 ml
» Sample buffer 41X, 1 ml
» TEMED, 300 μl
» APS (to reconstitute), 250 mg, final volumen 2.5 ml

Table for preparation of different resolution gels

 

15%

12%

10%

7.5%

Deionized water

2.45 ml

3.45 ml

4.15 ml

4.95 ml

Separation Buffer

2.5 ml

2.5 ml

2.5 ml

2.5 ml

Premix solution

5.0 ml

4.0 ml

3.3 ml

2.5 ml

APS solution

50 μl

50 μl

50 μl

50 μl

TEMED

5 μl

5 μl

5 μl

5 μl

 

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Blue-Kit
Quick-to-Use product

Bradford reagent for protein quantification. It is a sensible and accurate method.

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Quick-DNA
Quick-to-Use product

Reagent for DNA purification with phenol-chloroform-isoamilic solution 25:24:1

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Biolumina
Quick-to-Use product


Chemioluminiscent reagent ECL like for detection of HRP (HorseRadish Peroxidase), which is well known technology for Western Blots. The method is sensible and cost effective.

Advantages

» High sensible detection system, more than ten fold sensible than colorimetric systems to picogram detection.
» High resolution
» Quick protein detection

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Quick-Zol
Quick-to-Use product

Reagent for total RNA purification from different samples like blood, plants, bacteria, virus. Quick-Zol is an optimized version of standard purification protocol.
Is a highly reproducible method.

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Green-Kit, new!
Quick-to-Use product

Kit for lysis of resistent cells tissues (plants, fungy and yeast), with glass microparticles that gives quickness and efectiveness to the process of cellular disruption. It can then proceeed to recovery of proteins, DNA, RNA and organelles of interest.

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RNAsa Clean
Quick-to-Use product

Work place cleaning solution, also used for equipment and common use materials, that eliminates the presence of RNAse, it comes ready to use with spray type applicator.

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Version 1.4.5 - Last updated 3/26/2016
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